PhytophthoraV1, Main, Exploration, bibRecord, 000937

Proteomic Analysis of Phytophthora infestans Reveals the Importance of Cell Wall Proteins in Pathogenicity.

Identifieur interne : 000937 ( Main/Exploration ); précédent : 000936; suivant : 000938

Proteomic Analysis of Phytophthora infestans Reveals the Importance of Cell Wall Proteins in Pathogenicity.

Auteurs : Svante Resjö [Suède] ; Maja Brus [Suède] ; Ashfaq Ali [Suède] ; Harold J G. Meijer [Pays-Bas] ; Marianne Sandin [Suède] ; Francine Govers [Pays-Bas] ; Fredrik Levander [Suède] ; Laura Grenville-Briggs [Suède] ; Erik Andreasson [Suède]

Source :

Molecular & cellular proteomics : MCP [ 1535-9484 ] ; 2017.

RBID : pubmed:28935716

Descripteurs français

KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Facteurs de virulence (génétique), Facteurs de virulence (métabolisme), Maladies des plantes (parasitologie), Paroi cellulaire (métabolisme), Phytophthora infestans (croissance et développement), Phytophthora infestans (métabolisme), Phytophthora infestans (pathogénicité), Protéomique (méthodes), Régulation de l'expression des gènes au cours du développement (MeSH), Solanum tuberosum (parasitologie), Spectrométrie de masse (MeSH).
MESH :
- croissance et développement : Phytophthora infestans.
- génétique : Facteurs de virulence.
- métabolisme : Facteurs de virulence, Paroi cellulaire, Phytophthora infestans.
- méthodes : Protéomique.
- parasitologie : Maladies des plantes, Solanum tuberosum.
- pathogénicité : Phytophthora infestans.
- Analyse de profil d'expression de gènes, Régulation de l'expression des gènes au cours du développement, Spectrométrie de masse.

English descriptors

KwdEn :
- Cell Wall (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Developmental (MeSH), Mass Spectrometry (MeSH), Phytophthora infestans (growth & development), Phytophthora infestans (metabolism), Phytophthora infestans (pathogenicity), Plant Diseases (parasitology), Proteomics (methods), Solanum tuberosum (parasitology), Virulence Factors (genetics), Virulence Factors (metabolism).
MESH :
- chemical , genetics : Virulence Factors.
- growth & development : Phytophthora infestans.
- metabolism : Cell Wall, Phytophthora infestans, Virulence Factors.
- methods : Proteomics.
- parasitology : Plant Diseases, Solanum tuberosum.
- pathogenicity : Phytophthora infestans.
- Gene Expression Profiling, Gene Expression Regulation, Developmental, Mass Spectrometry.

Abstract

The oomycete Phytophthora infestans is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six P. infestans life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up- or downregulated in different combinations of life stages. One of these profiles contained 59 proteins that were more abundant in germinated cysts and appressoria. A large majority of these proteins were not previously recognized as being appressorial proteins or involved in the infection process. Among those are proteins with putative roles in transport, amino acid metabolism, pathogenicity (including one RXLR effector) and cell wall structure modification. We analyzed the expression of the genes encoding nine of these proteins using RT-qPCR and found an increase in transcript levels during disease progression, in agreement with the hypothesis that these proteins are important in early infection. Among the nine proteins was a group involved in cell wall structure modification and adhesion, including three closely related, uncharacterized proteins encoded by PITG_01131, PITG_01132, and PITG_16135, here denoted Piacwp1-3 Transient silencing of these genes resulted in reduced severity of infection, indicating that these proteins are important for pathogenicity. Our results contribute to further insight into P. infestans biology, and indicate processes that might be relevant for the pathogen while preparing for host cell penetration and during infection. The mass spectrometry data have been deposited to ProteomeXchange via the PRIDE partner repository with the data set identifier PXD002446.

DOI: 10.1074/mcp.M116.065656
PubMed: 28935716
PubMed Central: PMC5672002

Affiliations:

Pays-Bas, Suède

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000910
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Le document en format XML

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 Reveals the Importance of Cell Wall Proteins in Pathogenicity.</title>
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 Reveals the Importance of Cell Wall Proteins in Pathogenicity.</title>
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<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Developmental (MeSH)</term>
<term>Mass Spectrometry (MeSH)</term>
<term>Phytophthora infestans (growth & development)</term>
<term>Phytophthora infestans (metabolism)</term>
<term>Phytophthora infestans (pathogenicity)</term>
<term>Plant Diseases (parasitology)</term>
<term>Proteomics (methods)</term>
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<term>Virulence Factors (metabolism)</term>
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<term>Facteurs de virulence (métabolisme)</term>
<term>Maladies des plantes (parasitologie)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Phytophthora infestans (croissance et développement)</term>
<term>Phytophthora infestans (métabolisme)</term>
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<term>Phytophthora infestans</term>
<term>Virulence Factors</term>
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<term>Phytophthora infestans</term>
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<front><div type="abstract" xml:lang="en">The oomycete <i>Phytophthora infestans</i>
 is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six <i>P. infestans</i>
 life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up- or downregulated in different combinations of life stages. One of these profiles contained 59 proteins that were more abundant in germinated cysts and appressoria. A large majority of these proteins were not previously recognized as being appressorial proteins or involved in the infection process. Among those are proteins with putative roles in transport, amino acid metabolism, pathogenicity (including one RXLR effector) and cell wall structure modification. We analyzed the expression of the genes encoding nine of these proteins using RT-qPCR and found an increase in transcript levels during disease progression, in agreement with the hypothesis that these proteins are important in early infection. Among the nine proteins was a group involved in cell wall structure modification and adhesion, including three closely related, uncharacterized proteins encoded by PITG_01131, PITG_01132, and PITG_16135, here denoted <i>Piacwp1-3</i>
 Transient silencing of these genes resulted in reduced severity of infection, indicating that these proteins are important for pathogenicity. Our results contribute to further insight into <i>P. infestans</i>
 biology, and indicate processes that might be relevant for the pathogen while preparing for host cell penetration and during infection. The mass spectrometry data have been deposited to ProteomeXchange via the PRIDE partner repository with the data set identifier PXD002446.</div>
</front>
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<DateCompleted><Year>2018</Year>
<Month>06</Month>
<Day>19</Day>
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<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
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<Issue>11</Issue>
<PubDate><Year>2017</Year>
<Month>11</Month>
</PubDate>
</JournalIssue>
<Title>Molecular & cellular proteomics : MCP</Title>
<ISOAbbreviation>Mol Cell Proteomics</ISOAbbreviation>
</Journal>
<ArticleTitle>Proteomic Analysis of <i>Phytophthora infestans</i>
 Reveals the Importance of Cell Wall Proteins in Pathogenicity.</ArticleTitle>
<Pagination><MedlinePgn>1958-1971</MedlinePgn>
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<ELocationID EIdType="doi" ValidYN="Y">10.1074/mcp.M116.065656</ELocationID>
<Abstract><AbstractText>The oomycete <i>Phytophthora infestans</i>
 is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six <i>P. infestans</i>
 life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up- or downregulated in different combinations of life stages. One of these profiles contained 59 proteins that were more abundant in germinated cysts and appressoria. A large majority of these proteins were not previously recognized as being appressorial proteins or involved in the infection process. Among those are proteins with putative roles in transport, amino acid metabolism, pathogenicity (including one RXLR effector) and cell wall structure modification. We analyzed the expression of the genes encoding nine of these proteins using RT-qPCR and found an increase in transcript levels during disease progression, in agreement with the hypothesis that these proteins are important in early infection. Among the nine proteins was a group involved in cell wall structure modification and adhesion, including three closely related, uncharacterized proteins encoded by PITG_01131, PITG_01132, and PITG_16135, here denoted <i>Piacwp1-3</i>
 Transient silencing of these genes resulted in reduced severity of infection, indicating that these proteins are important for pathogenicity. Our results contribute to further insight into <i>P. infestans</i>
 biology, and indicate processes that might be relevant for the pathogen while preparing for host cell penetration and during infection. The mass spectrometry data have been deposited to ProteomeXchange via the PRIDE partner repository with the data set identifier PXD002446.</AbstractText>
<CopyrightInformation>© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Resjö</LastName>
<ForeName>Svante</ForeName>
<Initials>S</Initials>
<Identifier Source="ORCID">0000-0002-1622-3191</Identifier>
<AffiliationInfo><Affiliation>From the ‡Department of Plant Protection Biology, Swedish University of Agricultural Sciences, PO Box 102, SE-230 53 Alnarp, Sweden; svante.resjo@slu.se.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Brus</LastName>
<ForeName>Maja</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>From the ‡Department of Plant Protection Biology, Swedish University of Agricultural Sciences, PO Box 102, SE-230 53 Alnarp, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ali</LastName>
<ForeName>Ashfaq</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>From the ‡Department of Plant Protection Biology, Swedish University of Agricultural Sciences, PO Box 102, SE-230 53 Alnarp, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Meijer</LastName>
<ForeName>Harold J G</ForeName>
<Initials>HJG</Initials>
<AffiliationInfo><Affiliation>§Laboratory of Phytopathology, Wageningen University and Research, The Netherlands.</Affiliation>
</AffiliationInfo>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Mol Gen Genet. 1994 Aug 2;244(3):269-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8058038</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2015 Sep 21;10(9):e0137481</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26390127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2008 Mar;20(3):720-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18349153</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2008 Jan;7(1):225-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18173221</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Sep 17;461(7262):393-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19741609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2015 Jan;43(Database issue):D213-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25428371</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2008 Aug;7(8):1501-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18316789</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2012 Sep 15;28(18):2404-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22815360</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Signal. 2009 May 26;2(72):re3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19471024</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>CRC Crit Rev Biochem. 1988;23(3):283-337</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3069329</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Biol. 2010 Sep;114(9):702-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20943180</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Microbiol. 1968;22:87-108</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4879523</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2000 Dec;11(12):4241-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11102521</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2012 Feb;23(4):679-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22219373</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2013 Jan;41(Database issue):D1063-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23203882</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2006 Aug 1;22(15):1902-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16766559</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2014 Aug;13(8):2101-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24872595</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2008 Jan;7(1):29-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18067246</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gene. 1994 Jan 28;138(1-2):67-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8125319</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2005 Mar;18(3):229-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15782637</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2013 May;12(5):1407-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23306530</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2009 Aug 15;25(16):2028-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19535538</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2006 Jul;18(7):1766-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16766692</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2010 Nov 5;9(11):5748-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20831241</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gene. 1996 Nov 7;179(1):45-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8955628</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2003 Jul;14(7):2890-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12857872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2011 Mar;11(6):1114-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21298787</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2011 Jan;10(1):R110.000133</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20716697</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2005 Mar;42(3):244-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15707845</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2009 Jun;8(6):3037-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19354269</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2007 Mar;4(3):207-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17327847</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Microbiol. 2008 Nov;10(11):2271-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18637942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2014 Apr 4;13(4):1848-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24588563</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Genet. 2005 May;47(5):307-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15815927</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2008 Nov 1;24(21):2534-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18606607</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2003 Oct;40(1):4-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12948509</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Microbiol. 2005 Jan;3(1):47-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15608699</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2003 Aug 22;278(34):32107-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12764138</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1990 Jul;87(14):5331-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2142530</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2003 Feb 12;19(3):368-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12584122</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2015 Jan;43(Database issue):D204-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25348405</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 2007 Oct;66(2):534-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17854403</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2006 Dec;19(12):1348-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17153919</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cold Spring Harb Perspect Biol. 2014 May 01;6(5):a016147</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24789819</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2008 Apr;21(4):433-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18321189</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Pays-Bas</li>
<li>Suède</li>
</country>
</list>
<tree><country name="Suède"><noRegion><name sortKey="Resjo, Svante" sort="Resjo, Svante" uniqKey="Resjo S" first="Svante" last="Resjö">Svante Resjö</name>
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<name sortKey="Sandin, Marianne" sort="Sandin, Marianne" uniqKey="Sandin M" first="Marianne" last="Sandin">Marianne Sandin</name>
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<country name="Pays-Bas"><noRegion><name sortKey="Meijer, Harold J G" sort="Meijer, Harold J G" uniqKey="Meijer H" first="Harold J G" last="Meijer">Harold J G. Meijer</name>
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Proteomic Analysis of Phytophthora infestans Reveals the Importance of Cell Wall Proteins in Pathogenicity.

Proteomic Analysis of Phytophthora infestans Reveals the Importance of Cell Wall Proteins in Pathogenicity.

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